Description
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of sialic acid in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to sialic acid has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled sialic acid and unlabeled sialic acid (Standards or samples) with the pre-coated antibody specific to sialic acid. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of sialic acid in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of sialic acid in the sample